Hotspot

ycf4-cemA

LSC · 57997-58538 · 542 bp

Back to Pseudostellaria

Pi 0.027

MarkerSeek score 63.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 34
pi: 0.027
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CTCTTCCTTTGACCCGTACG	ATAGCATGATCCACCAGGGT	55.0	50.0	58.0	58.2	715-731 bp	1.0	86.2
ycf4-cemA_p2	TAAGTAATTTCGGCTGGGCC	ATAGCATGATCCACCAGGGT	50.0	50.0	58.0	58.2	1110-1127 bp	1.0	84.5
ycf4-cemA_p3	ATGAGAGTTACGCCGGAAAC	ATAGCATGATCCACCAGGGT	50.0	50.0	58.0	58.2	1258-1275 bp	1.0	84.1
ycf4-cemA_p4	GAGAGTTACGCCGGAAACAT	ATAGCATGATCCACCAGGGT	50.0	50.0	58.0	58.2	1256-1273 bp	1.0	84.1
ycf4-cemA_p5	TGAATTGGCGATCAGAACGT	ATAGCATGATCCACCAGGGT	45.0	50.0	57.9	58.2	1164-1181 bp	1.0	83.9