Hotspot

trnT-GGU-psbD

LSC · 30417-31488 · 1072 bp

Back to Pseudostellaria

Pi 0.011

MarkerSeek score 63.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 31
pi: 0.011
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	ACGGTCTCTACGTAACCAGT	55.0	50.0	58.1	57.8	1092-1232 bp	1.0	84.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.1	57.8	1095-1235 bp	1.0	83.8
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.3	57.8	1069-1209 bp	1.0	82.3
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	ACGGTCTCTACGTAACCAGT	50.0	50.0	57.7	57.8	1107-1247 bp	1.0	82.2
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	ACGGTCTCTACGTAACCAGT	50.0	50.0	58.4	57.8	1091-1231 bp	1.0	81.0