Hotspot

trnS-GCU-trnG-UCC

LSC · 8060-8769 · 710 bp

Back to Pseudostellaria

Pi 0.0166

MarkerSeek score 69.20

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 710
variable_sites: 32
pi: 0.0166
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CACGGAAAGAGAGGGATTCG	CGGAATAGTAATCAAACCGGGA	55.0	45.5	58.1	57.9	844-994 bp	1.0	69.4
trnS-GCU-trnG-UCC_p2	CACGGAAAGAGAGGGATTCG	TCGGAATAGTAATCAAACCGGG	55.0	45.5	58.1	57.9	845-995 bp	1.0	69.4
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	CGGAATAGTAATCAAACCGGGA	50.0	45.5	58.2	57.9	843-993 bp	1.0	68.8
trnS-GCU-trnG-UCC_p4	ACGGAAAGAGAGGGATTCGA	TCGGAATAGTAATCAAACCGGG	50.0	45.5	58.2	57.9	844-994 bp	1.0	68.8
trnS-GCU-trnG-UCC_p5	CACGGAAAGAGAGGGATTCG	CGGAATAGTAATCAAACCGGG	55.0	47.6	58.1	56.5	844-994 bp	1.0	65.8