Hotspot

psbI-trnS-GCU

LSC · 7812-7971 · 160 bp

Back to Pseudostellaria

Pi 0.0424

MarkerSeek score 79.60

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 160
variable_sites: 15
pi: 0.0424
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	AAGAGGGAGAGATGGCTGAG	50.0	55.0	58.3	58.2	287-294 bp	1.0	80.9
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	TTCAAGAGGGAGAGATGGCT	50.0	50.0	58.3	57.7	290-297 bp	1.0	80.4
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	CGAGTTATTCGTACCGAGGG	50.0	55.0	58.3	57.6	234-241 bp	1.0	80.4
psbI-trnS-GCU_p4	ATGATCCAGGGCGTAATCCT	CAAGAGGGAGAGATGGCTGA	50.0	55.0	58.3	58.5	288-295 bp	1.0	78.5
psbI-trnS-GCU_p5	ATGATCCAGGGCGTAATCCT	TCAAGAGGGAGAGATGGCTG	50.0	55.0	58.3	58.5	289-296 bp	1.0	78.5