Hotspot

trnK-UUU-rps16

LSC · 4209-4846 · 638 bp

Back to Pseudostellaria

Pi 0.00836
MarkerSeek score 61.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
638
variable_sites
15
pi
0.00836
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 814-823 bp 1.0 81.4
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC AAAAAGGCGCTCAACCTACA 50.0 45.0 58.0 58.0 815-824 bp 1.0 81.4
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 766-775 bp 1.0 80.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAAAAGGCGCTCAACCTACA 50.0 45.0 58.2 58.0 765-774 bp 1.0 80.5
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA GCGCTCAACCTACAGAAACT 50.0 50.0 58.0 58.2 808-817 bp 1.0 80.0