Hotspot

thiG

IRa · 88041-88850 · 810 bp

Back to Pseudostaurastrum

Pi 0.0901
MarkerSeek score 79.80
Primer available yes
Label thiG

Feature Metadata

label_name
thiG
feature_type
gene
region
IRa
length_bp
810
variable_sites
73
pi
0.0901
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
thiG_p1 AATGGTTGTAAAGCTGGGCA TGACAGCAAAACTCAGTCGT 45.0 45.0 58.0 57.7 1562 bp 0.5 57.5
thiG_p2 ATGGTTGTAAAGCTGGGCAA TGACAGCAAAACTCAGTCGT 45.0 45.0 58.0 57.7 1561 bp 0.5 57.5
thiG_p3 AATGGTTGTAAAGCTGGGCA AGTGCGTCAAAGAGGAACAA 45.0 45.0 58.0 57.7 1766 bp 0.5 57.3
thiG_p4 ATGGTTGTAAAGCTGGGCAA AGTGCGTCAAAGAGGAACAA 45.0 45.0 58.0 57.7 1765 bp 0.5 57.3
thiG_p5 GGGTAGCCCTTAGGAAAAGC TGACAGCAAAACTCAGTCGT 55.0 45.0 57.9 57.7 1764 bp 0.5 57.1
thiG_p1 TGACAGCAAAACTCAGTCGT AATGGTTGTAAAGCTGGGCA 45.0 45.0 57.7 58.0 1562 bp 0.5 57.5
thiG_p2 TGACAGCAAAACTCAGTCGT ATGGTTGTAAAGCTGGGCAA 45.0 45.0 57.7 58.0 1561 bp 0.5 57.5
thiG_p3 TGACAGCAAAACTCAGTCGT GGGTAGCCCTTAGGAAAAGC 45.0 55.0 57.7 57.9 1764 bp 0.5 57.1
thiG_p4 TTAGACGCGCAAAGCTCTAC AATGGTTGTAAAGCTGGGCA 50.0 45.0 58.4 58.0 1240 bp 0.5 56.9
thiG_p5 TTAGACGCGCAAAGCTCTAC ATGGTTGTAAAGCTGGGCAA 50.0 45.0 58.4 58.0 1239 bp 0.5 56.9