Hotspot

rpoC1

LSC · 61140-63473 · 2334 bp

Back to Pseudostaurastrum

Pi 0.175

MarkerSeek score 77.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2334
variable_sites: 409
pi: 0.175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GCGTAACAAAACCCAGCTTC	AGATGATACACCTGGGCGTA	50.0	50.0	58.0	57.9	2939 bp	0.5	59.0
rpoC1_p2	GCGTAACAAAACCCAGCTTC	GGCACTAGAAGCGTATGGAG	50.0	55.0	58.0	57.9	2996 bp	0.5	58.5
rpoC1_p3	GCGTAACAAAACCCAGCTTC	GATACCGAAGCCAGGTGTAC	50.0	55.0	58.0	57.8	2885 bp	0.5	57.9
rpoC1_p4	GCGTAACAAAACCCAGCTTC	CTAGAAGCGTATGGAGCAGC	50.0	55.0	58.0	58.2	2992 bp	0.5	57.8
rpoC1_p5	AGATACCACCTTGTGTTGCG	AGATGATACACCTGGGCGTA	50.0	50.0	58.2	57.9	2956 bp	0.5	57.8