Hotspot

orf1_gon

LSC · 34445-35440 · 996 bp

Back to Pseudostaurastrum

Pi 0.244

MarkerSeek score 79.30

Primer available yes

Label orf1_gon

Feature Metadata

label_name: orf1_gon
feature_type: gene
region: LSC
length_bp: 996
variable_sites: 242
pi: 0.244
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf1_gon_p1	TCTGCAGTTTGGATTTGTTGT	CCTTCGCCGGTTTTCATTTC	38.1	50.0	56.6	58.0	1607 bp	0.5	40.2
orf1_gon_p2	TCTGCAGTTTGGATTTGTTGT	TGCGGTTGAATGACGTGTTA	38.1	45.0	56.6	58.1	1515 bp	0.5	39.4
orf1_gon_p3	TCTGCAGTTTGGATTTGTTGT	GCCGGTTTTCATTTCAGCAA	38.1	45.0	56.6	57.9	1602 bp	0.5	39.2
orf1_gon_p4	TCTGCAGTTTGGATTTGTTGT	TAACTGCGGTTGAATGACGT	38.1	45.0	56.6	57.8	1519 bp	0.5	39.2
orf1_gon_p5	TCTGCAGTTTGGATTTGTTGT	GACGAAAAACACCACCCAAC	38.1	50.0	56.6	57.8	1327 bp	0.5	38.8