Hotspot

lysR

LSC · 41618-42445 · 828 bp

Back to Pseudostaurastrum

Pi 0.335

MarkerSeek score 82.40

Primer available yes

Label lysR

Feature Metadata

label_name: lysR
feature_type: gene
region: LSC
length_bp: 828
variable_sites: 268
pi: 0.335
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
lysR_p1	CAGTTGTACCGGGTTACAGA	AACTGCCGGTTCCAAAATCT	50.0	45.0	56.9	58.0	1921 bp	0.5	51.1
lysR_p2	CAGTTGTACCGGGTTACAGA	ACTGCCGGTTCCAAAATCTT	50.0	45.0	56.9	58.0	1920 bp	0.5	51.1
lysR_p3	TCAGTTGTACCGGGTTACAG	AACTGCCGGTTCCAAAATCT	50.0	45.0	56.9	58.0	1922 bp	0.5	51.1
lysR_p4	TCAGTTGTACCGGGTTACAG	ACTGCCGGTTCCAAAATCTT	50.0	45.0	56.9	58.0	1921 bp	0.5	51.1
lysR_p5	CAGTTGTACCGGGTTACAGA	GTACAAAAATGGGCCGAACG	50.0	50.0	56.9	58.0	2168 bp	0.5	50.9