Hotspot

rns-trnI(gat)

IRb · 26964-27031 · 68 bp

Back to Pseudostaurastrum

Pi 0.0714

MarkerSeek score 81.20

Primer available yes

Label rns-trnI(gat)

Feature Metadata

label_name: rns-trnI(gat)
feature_type: igs
region: IRb
length_bp: 68
variable_sites: 4
pi: 0.0714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-trnI(gat)_p1	GACCGAGGTGAAGTCGTAAC	TACCAGCTGAGCTATACCCC	55.0	55.0	58.0	58.0	215-227 bp	1.0	85.2
rns-trnI(gat)_p2	GACCGAGGTGAAGTCGTAAC	TGGACTTGAACCAGAGACCT	55.0	50.0	58.0	57.9	181-193 bp	1.0	84.7
rns-trnI(gat)_p3	GACCGAGGTGAAGTCGTAAC	GCTGGGCTATTCTGGACTTG	55.0	55.0	58.0	58.3	193-205 bp	1.0	82.8
rns-trnI(gat)_p4	AAGTCGTAACAAGGTAGCCG	TACCAGCTGAGCTATACCCC	50.0	55.0	57.6	58.0	205-217 bp	1.0	82.6
rns-trnI(gat)_p5	TGACCGAGGTGAAGTCGTAA	TACCAGCTGAGCTATACCCC	50.0	55.0	58.4	58.0	216-228 bp	1.0	82.2