primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps5_p1	rps5	1	TCTTCTTCTGCATCTTGCCG	AGATTCGTGCAATTCGTCCA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.362805	14418	15047	1383-1383	0.500000	57.1
rps5_p2	rps5	2	TCTTCTTCTGCATCTTGCCG	CAATTCGTCCACCTGAACCA	20	20	50.0	50.0	58.3	58.1	0.0	0.0	0.378623	14418	15047	1374-1374	0.500000	57.0
rps5_p3	rps5	3	TCTTCTTCTGCATCTTGCCG	GATTCGTGCAATTCGTCCAC	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.428966	14418	15047	1382-1382	0.500000	56.6
rps5_p4	rps5	4	TCTTCTTCTGCATCTTGCCG	TCGTCCACCTGAACCATACA	20	20	50.0	50.0	58.3	58.4	0.0	0.0	0.634539	14418	15047	1370-1370	0.500000	54.9
rps5_p5	rps5	5	TCTTCTTCTGCATCTTGCCG	CGTCCACCTGAACCATACAA	20	20	50.0	50.0	58.3	57.5	0.0	0.0	0.735359	14418	15047	1369-1369	0.500000	54.1
rns-trnI(gat)_p1	rns-trnI(gat)	1	GACCGAGGTGAAGTCGTAAC	TACCAGCTGAGCTATACCCC	20	20	55.0	55.0	58.0	58.0	0.0	17.4	0.036636	26964	27031	215-227	1.000000	85.2
rns-trnI(gat)_p2	rns-trnI(gat)	2	GACCGAGGTGAAGTCGTAAC	TGGACTTGAACCAGAGACCT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.128667	26964	27031	181-193	1.000000	84.7
rns-trnI(gat)_p3	rns-trnI(gat)	3	GACCGAGGTGAAGTCGTAAC	GCTGGGCTATTCTGGACTTG	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.352345	26964	27031	193-205	1.000000	82.8
rns-trnI(gat)_p4	rns-trnI(gat)	4	AAGTCGTAACAAGGTAGCCG	TACCAGCTGAGCTATACCCC	20	20	50.0	55.0	57.6	58.0	0.0	17.4	0.370061	26964	27031	205-217	1.000000	82.6
rns-trnI(gat)_p5	rns-trnI(gat)	5	TGACCGAGGTGAAGTCGTAA	TACCAGCTGAGCTATACCCC	20	20	50.0	55.0	58.4	58.0	0.0	17.4	0.403068	26964	27031	216-228	1.000000	82.2
thiG_p1	thiG	1	AATGGTTGTAAAGCTGGGCA	TGACAGCAAAACTCAGTCGT	20	20	45.0	45.0	58.0	57.7	0.0	0.0	0.317770	30847	31656	1562-1562	0.500000	57.5
thiG_p2	thiG	2	ATGGTTGTAAAGCTGGGCAA	TGACAGCAAAACTCAGTCGT	20	20	45.0	45.0	58.0	57.7	0.0	0.0	0.317770	30847	31656	1561-1561	0.500000	57.5
thiG_p3	thiG	3	AATGGTTGTAAAGCTGGGCA	AGTGCGTCAAAGAGGAACAA	20	20	45.0	45.0	58.0	57.7	0.0	1.2	0.333825	30847	31656	1766-1766	0.500000	57.3
thiG_p4	thiG	4	ATGGTTGTAAAGCTGGGCAA	AGTGCGTCAAAGAGGAACAA	20	20	45.0	45.0	58.0	57.7	0.0	1.2	0.333825	30847	31656	1765-1765	0.500000	57.3
thiG_p5	thiG	5	GGGTAGCCCTTAGGAAAAGC	TGACAGCAAAACTCAGTCGT	20	20	55.0	45.0	57.9	57.7	0.0	0.0	0.363760	30847	31656	1764-1764	0.500000	57.1
ycf60_p1	ycf60	1	TTGATCCAAGGCCTTCATCG	ACTGGAGATCTTGATGCTTGT	20	21	50.0	42.9	58.0	57.0	17.3	0.0	2.039607	33200	34393	2262-2262	0.500000	43.7
ycf60_p2	ycf60	2	ATAAAAACTCACGGCTGCCA	ACTGGAGATCTTGATGCTTGT	20	21	45.0	42.9	58.1	57.0	20.3	0.0	2.092634	33200	34393	1699-1699	0.500000	43.3
ycf60_p3	ycf60	3	TCTGCCACACGCTTCTAAAA	ACTGGAGATCTTGATGCTTGT	20	21	45.0	42.9	57.8	57.0	0.0	0.0	2.245201	33200	34393	2060-2060	0.500000	42.0
ycf60_p4	ycf60	4	AACGTGTTGGCTCAATCCAA	ACTGGAGATCTTGATGCTTGT	20	21	45.0	42.9	58.3	57.0	0.0	0.0	2.310566	33200	34393	2085-2085	0.500000	41.5
ycf60_p5	ycf60	5	ACGTGTTGGCTCAATCCAAA	ACTGGAGATCTTGATGCTTGT	20	21	45.0	42.9	58.3	57.0	0.0	0.0	2.310566	33200	34393	2084-2084	0.500000	41.5
orf1_gon_p1	orf1_gon	1	TCTGCAGTTTGGATTTGTTGT	CCTTCGCCGGTTTTCATTTC	21	20	38.1	50.0	56.6	58.0	14.7	1.9	2.469563	34445	35440	1607-1607	0.500000	40.2
orf1_gon_p2	orf1_gon	2	TCTGCAGTTTGGATTTGTTGT	TGCGGTTGAATGACGTGTTA	21	20	38.1	45.0	56.6	58.1	14.7	0.0	2.579585	34445	35440	1515-1515	0.500000	39.4
orf1_gon_p3	orf1_gon	3	TCTGCAGTTTGGATTTGTTGT	GCCGGTTTTCATTTCAGCAA	21	20	38.1	45.0	56.6	57.9	14.7	1.9	2.594187	34445	35440	1602-1602	0.500000	39.2
orf1_gon_p4	orf1_gon	4	TCTGCAGTTTGGATTTGTTGT	TAACTGCGGTTGAATGACGT	21	20	38.1	45.0	56.6	57.8	14.7	21.4	2.604585	34445	35440	1519-1519	0.500000	39.2
orf1_gon_p5	orf1_gon	5	TCTGCAGTTTGGATTTGTTGT	GACGAAAAACACCACCCAAC	21	20	38.1	50.0	56.6	57.8	14.7	0.0	2.644543	34445	35440	1327-1327	0.500000	38.8
lysR_p1	lysR	1	CAGTTGTACCGGGTTACAGA	AACTGCCGGTTCCAAAATCT	20	20	50.0	45.0	56.9	58.0	4.3	1.9	1.118081	41618	42445	1921-1921	0.500000	51.1
lysR_p2	lysR	2	CAGTTGTACCGGGTTACAGA	ACTGCCGGTTCCAAAATCTT	20	20	50.0	45.0	56.9	58.0	4.3	1.9	1.118081	41618	42445	1920-1920	0.500000	51.1
lysR_p3	lysR	3	TCAGTTGTACCGGGTTACAG	AACTGCCGGTTCCAAAATCT	20	20	50.0	45.0	56.9	58.0	4.3	1.9	1.118081	41618	42445	1922-1922	0.500000	51.1
lysR_p4	lysR	4	TCAGTTGTACCGGGTTACAG	ACTGCCGGTTCCAAAATCTT	20	20	50.0	45.0	56.9	58.0	4.3	1.9	1.118081	41618	42445	1921-1921	0.500000	51.1
lysR_p5	lysR	5	CAGTTGTACCGGGTTACAGA	GTACAAAAATGGGCCGAACG	20	20	50.0	50.0	56.9	58.0	4.3	0.0	1.141665	41618	42445	2168-2168	0.500000	50.9
ycf12-rbcS_p1	ycf12-rbcS	1	AGTTGACTAAGAACCTGCAA	TCCTCCTGGAGAAAGATACGT	20	21	40.0	47.6	54.5	57.6	0.0	25.2	4.872846	47864	48017	202-208	1.000000	50.8
ycf12-rbcS_p2	ycf12-rbcS	2	AAGTTGACTAAGAACCTGCA	TCCTCCTGGAGAAAGATACGT	20	21	40.0	47.6	54.5	57.6	0.0	25.2	4.872846	47864	48017	203-209	1.000000	50.7
ycf12-rbcS_p3	ycf12-rbcS	3	AGTTGACTAAGAACCTGCAAA	TCCTCCTGGAGAAAGATACGT	21	21	38.1	47.6	55.4	57.6	0.0	25.2	5.025996	47864	48017	202-208	1.000000	49.8
ycf12-rbcS_p4	ycf12-rbcS	4	AAGTTGACTAAGAACCTGCAA	TCCTCCTGGAGAAAGATACGT	21	21	38.1	47.6	55.4	57.6	0.0	25.2	5.025996	47864	48017	203-209	1.000000	49.7
ycf12-rbcS_p5	ycf12-rbcS	5	ACGCAATAAGTTGACTAAGAACC	TCCTCCTGGAGAAAGATACGT	23	21	39.1	47.6	57.1	57.6	0.0	25.2	5.241520	47864	48017	210-216	1.000000	49.4
rpoC2_p1	rpoC2	1	ATGACCAGAATCGGCTGTTC	GCTTTCAACGCGGATTTTGA	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.074726	60868	60987	1349-1349	0.500000	59.4
rpoC2_p2	rpoC2	2	ATGACCAGAATCGGCTGTTC	AGCTTTCAACGCGGATTTTG	20	20	50.0	45.0	58.0	57.9	0.0	12.5	0.080315	60868	60987	1350-1350	0.500000	59.4
rpoC2_p3	rpoC2	3	ATGACCAGAATCGGCTGTTC	CAGCTTTCAACGCGGATTTT	20	20	50.0	45.0	58.0	57.9	0.0	12.5	0.080315	60868	60987	1351-1351	0.500000	59.4
rpoC2_p4	rpoC2	4	GCGTAACAAAACCCAGCTTC	GCTTTCAACGCGGATTTTGA	20	20	50.0	45.0	58.0	58.0	0.0	12.5	0.091170	60868	60987	895-895	0.500000	59.3
rpoC2_p5	rpoC2	5	CATTAGTCCCCGCATTCCAA	GCTTTCAACGCGGATTTTGA	20	20	50.0	45.0	57.9	58.0	0.0	12.5	0.096222	60868	60987	1205-1205	0.500000	59.2
rpoC1_p1	rpoC1	1	GCGTAACAAAACCCAGCTTC	AGATGATACACCTGGGCGTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.128423	61140	63473	2939-2939	0.500000	59.0
rpoC1_p2	rpoC1	2	GCGTAACAAAACCCAGCTTC	GGCACTAGAAGCGTATGGAG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.187818	61140	63473	2996-2996	0.500000	58.5
rpoC1_p3	rpoC1	3	GCGTAACAAAACCCAGCTTC	GATACCGAAGCCAGGTGTAC	20	20	50.0	55.0	58.0	57.8	0.0	5.7	0.266761	61140	63473	2885-2885	0.500000	57.9
rpoC1_p4	rpoC1	4	GCGTAACAAAACCCAGCTTC	CTAGAAGCGTATGGAGCAGC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.269182	61140	63473	2992-2992	0.500000	57.8
rpoC1_p5	rpoC1	5	AGATACCACCTTGTGTTGCG	AGATGATACACCTGGGCGTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.273729	61140	63473	2956-2956	0.500000	57.8
atpB_p1	atpB	1	GCCGTCAAGAAAAAGAAGCC	ATTGGTATCCAACGCCCTTC	20	20	50.0	50.0	57.9	57.9	0.0	3.2	0.104661	68240	69721	2168-2168	0.500000	59.2
atpB_p2	atpB	2	GCCGTCAAGAAAAAGAAGCC	GGTATCCAACGCCCTTCAAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.104661	68240	69721	2165-2165	0.500000	59.2
atpB_p3	atpB	3	GCCGTCAAGAAAAAGAAGCC	CTCCGCTTAAGGTAGGGAGA	20	20	50.0	55.0	57.9	57.9	0.0	4.3	0.120713	68240	69721	2077-2077	0.500000	59.0
atpB_p4	atpB	4	GCCGTCAAGAAAAAGAAGCC	TCTCCGCTTAAGGTAGGGAG	20	20	50.0	55.0	57.9	57.9	0.0	4.3	0.120713	68240	69721	2078-2078	0.500000	59.0
atpB_p5	atpB	5	GCCGTCAAGAAAAAGAAGCC	GGAGAACTGCTTGCTGAACT	20	20	50.0	50.0	57.9	58.1	0.0	6.5	0.162334	68240	69721	2062-2062	0.500000	58.7
ycf19_p1	ycf19	1	AACTGTAGATGATGGCTGCG	AATTGACGTGCGGCTAAGAT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.078788	78191	78820	1511-1511	0.500000	59.4
ycf19_p2	ycf19	2	AACTGTAGATGATGGCTGCG	CCGTCGTTCGCCCTTTTATA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.129383	78191	78820	974-974	0.500000	59.0
ycf19_p3	ycf19	3	GACCAAAACCATTGCCCAAC	AATTGACGTGCGGCTAAGAT	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.146539	78191	78820	1646-1646	0.500000	58.8
ycf19_p4	ycf19	4	CCCAAACCACCATGGCTAAT	AATTGACGTGCGGCTAAGAT	20	20	50.0	45.0	58.1	58.0	13.0	0.0	0.159507	78191	78820	1680-1680	0.500000	58.7
ycf19_p5	ycf19	5	GACCAAAACCATTGCCCAAC	CCGTCGTTCGCCCTTTTATA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.197134	78191	78820	1109-1109	0.500000	58.4
groEL_p1	groEL	1	CCCTCCTAGCTCGTCCTTAA	GGTCGTGTTCCATTATGGCT	20	20	55.0	50.0	57.9	58.0	0.0	6.3	0.107221	80424	82010	2115-2161	1.000000	89.5
groEL_p2	groEL	2	CCCTCCTAGCTCGTCCTTAA	TATGACAAGTGCTGGTCGTG	20	20	55.0	50.0	57.9	57.9	0.0	10.8	0.147683	80424	82010	2128-2174	1.000000	89.2
groEL_p3	groEL	3	CCCTCCTAGCTCGTCCTTAA	AGCGGTTCCAACTGTTTTCT	20	20	55.0	45.0	57.9	57.9	0.0	0.3	0.121617	80424	82010	2404-2439	1.000000	89.1
groEL_p4	groEL	4	CCCTCCTAGCTCGTCCTTAA	TGGATTAGCGGTTCCAACTG	20	20	55.0	50.0	57.9	57.9	0.0	5.6	0.176784	80424	82010	2445-2445	0.500000	58.6
groEL_p5	groEL	5	CCCTCCTAGCTCGTCCTTAA	GGATTAGCGGTTCCAACTGT	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.178259	80424	82010	2444-2444	0.500000	58.6
thiG_p1	thiG	1	TGACAGCAAAACTCAGTCGT	AATGGTTGTAAAGCTGGGCA	20	20	45.0	45.0	57.7	58.0	0.0	0.0	0.317770	88041	88850	1562-1562	0.500000	57.5
thiG_p2	thiG	2	TGACAGCAAAACTCAGTCGT	ATGGTTGTAAAGCTGGGCAA	20	20	45.0	45.0	57.7	58.0	0.0	0.0	0.317770	88041	88850	1561-1561	0.500000	57.5
thiG_p3	thiG	3	TGACAGCAAAACTCAGTCGT	GGGTAGCCCTTAGGAAAAGC	20	20	45.0	55.0	57.7	57.9	0.0	0.0	0.363760	88041	88850	1764-1764	0.500000	57.1
thiG_p4	thiG	4	TTAGACGCGCAAAGCTCTAC	AATGGTTGTAAAGCTGGGCA	20	20	50.0	45.0	58.4	58.0	5.7	0.0	0.382627	88041	88850	1240-1240	0.500000	56.9
thiG_p5	thiG	5	TTAGACGCGCAAAGCTCTAC	ATGGTTGTAAAGCTGGGCAA	20	20	50.0	45.0	58.4	58.0	5.7	0.0	0.382627	88041	88850	1239-1239	0.500000	56.9
trnI(gat)-rns_p1	trnI(gat)-rns	1	TACCAGCTGAGCTATACCCC	GACCGAGGTGAAGTCGTAAC	20	20	55.0	55.0	58.0	58.0	17.4	0.0	0.036636	92666	92733	215-227	1.000000	85.2
trnI(gat)-rns_p2	trnI(gat)-rns	2	TGGACTTGAACCAGAGACCT	GACCGAGGTGAAGTCGTAAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.128667	92666	92733	181-193	1.000000	84.7
trnI(gat)-rns_p3	trnI(gat)-rns	3	GCTGGGCTATTCTGGACTTG	GACCGAGGTGAAGTCGTAAC	20	20	55.0	55.0	58.3	58.0	0.0	0.0	0.352345	92666	92733	193-205	1.000000	82.8
trnI(gat)-rns_p4	trnI(gat)-rns	4	TACCAGCTGAGCTATACCCC	AAGTCGTAACAAGGTAGCCG	20	20	55.0	50.0	58.0	57.6	17.4	0.0	0.370061	92666	92733	205-217	1.000000	82.6
trnI(gat)-rns_p5	trnI(gat)-rns	5	TACCAGCTGAGCTATACCCC	TGACCGAGGTGAAGTCGTAA	20	20	55.0	50.0	58.0	58.4	17.4	0.0	0.403068	92666	92733	216-228	1.000000	82.2
clpN_p1	clpN	1	TAGCTGGAAAAAGTGCGGTT	GGCGAAACTCTTCTAGGCAA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.119103	107151	107672	1590-1590	0.500000	59.0
clpN_p2	clpN	2	TAGCTGGAAAAAGTGCGGTT	TTGGCGAAACTCTTCTAGGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.119103	107151	107672	1592-1592	0.500000	59.0
clpN_p3	clpN	3	TTAGCTGGAAAAAGTGCGGT	GGCGAAACTCTTCTAGGCAA	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.119103	107151	107672	1591-1591	0.500000	59.0
clpN_p4	clpN	4	TTAGCTGGAAAAAGTGCGGT	TTGGCGAAACTCTTCTAGGC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.119103	107151	107672	1593-1593	0.500000	59.0
clpN_p5	clpN	5	TAGCTGGAAAAAGTGCGGTT	CACAACCCTCAGCTAGCAAA	20	20	45.0	50.0	58.0	58.1	0.0	18.8	0.128121	107151	107672	1785-1785	0.500000	59.0
chlI_p1	chlI	1	TTGAATGATTGGCAACGTGC	TCCTGGGTGGTGAAACAATG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.069200	109124	110308	2263-2263	0.500000	59.4
chlI_p2	chlI	2	TTGAATGATTGGCAACGTGC	TATGTGTCACCACGCTCATG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.071907	109124	110308	2057-2057	0.500000	59.4
chlI_p3	chlI	3	TTGAATGATTGGCAACGTGC	CGCACTTCTTCCTAGGGTTC	20	20	45.0	55.0	57.9	58.0	0.0	9.5	0.074696	109124	110308	2026-2026	0.500000	59.4
chlI_p4	chlI	4	ACTTCAGGGATGGCAAAGTG	TCCTGGGTGGTGAAACAATG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.090903	109124	110308	2330-2330	0.500000	59.3
chlI_p5	chlI	5	ACTTCAGGGATGGCAAAGTG	TATGTGTCACCACGCTCATG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.093611	109124	110308	2124-2124	0.500000	59.3
trnF(gaa)-trnC(gca)_p1	trnF(gaa)-trnC(gca)	1	GCGACACATGGATCTTCAGT	TTTGCAATCCCCTGCCTTAC	20	20	50.0	50.0	58.0	58.4	0.0	0.0	0.459769	113544	113662	199-206	1.000000	88.3
trnF(gaa)-trnC(gca)_p2	trnF(gaa)-trnC(gca)	2	GCGACACATGGATCTTCAGT	ATTTGCAATCCCCTGCCTTA	20	20	50.0	45.0	58.0	57.5	0.0	0.0	0.564199	113544	113662	200-207	1.000000	87.4
trnF(gaa)-trnC(gca)_p3	trnF(gaa)-trnC(gca)	3	GCGACACATGGATCTTCAGT	TTACCACTTGGCTATACCGC	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.623367	113544	113662	183-190	1.000000	87.0
trnF(gaa)-trnC(gca)_p4	trnF(gaa)-trnC(gca)	4	GCGACACATGGATCTTCAGT	AATCCCCTGCCTTACCACTT	20	20	50.0	50.0	58.0	58.6	0.0	0.0	0.632821	113544	113662	194-201	1.000000	86.9
trnF(gaa)-trnC(gca)_p5	trnF(gaa)-trnC(gca)	5	CCAGAGCCAGACTTGAACTG	TTTGCAATCCCCTGCCTTAC	20	20	55.0	50.0	58.2	58.4	0.0	0.0	0.638164	113544	113662	219-226	1.000000	86.5