Hotspot

ycf1

SSC · 120731-125938 · 5208 bp

Back to Pseudosedum

Pi 0.0834

MarkerSeek score 71.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5208
variable_sites: 430
pi: 0.0834
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTAAAACTGCGGCTTTGTGC	TGGGCCGAGGATTTTGAAAA	45.0	45.0	58.2	58.0	1091 bp	0.5	58.8
ycf1_p2	TTAAAACTGCGGCTTTGTGC	GAACTAGCAGCAGAACCTCC	45.0	55.0	58.2	58.0	1247 bp	0.5	58.6
ycf1_p3	TTAAAACTGCGGCTTTGTGC	ACCCGAATTCCCTCAAATGG	45.0	50.0	58.2	57.9	930 bp	0.5	57.6
ycf1_p4	TTAAAACTGCGGCTTTGTGC	CCCCAAAACCAACCTTCCTT	45.0	50.0	58.2	58.2	824 bp	0.5	57.2
ycf1_p5	CTGCGGCTTTGTGCTAATTT	TGGGCCGAGGATTTTGAAAA	45.0	45.0	57.6	58.0	1085 bp	0.5	57.2