Hotspot

rpl32-trnL-UAG

SSC · 111039-111520 · 482 bp

Back to Pseudosedum

Pi 0.107

MarkerSeek score 81.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 482
variable_sites: 49
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	TTCGAACCGAGATGCTCAAG	50.0	50.0	58.0	58.0	627-639 bp	1.0	89.5
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCGTTAA	TTAGAAGAGAATGTGCCGCC	50.0	50.0	58.0	58.0	654-666 bp	1.0	88.9
rpl32-trnL-UAG_p3	GGGATATTGGGCAGCGTTAA	AGACTCGAAGTGGCAAATCC	50.0	50.0	58.0	57.9	1057-1068 bp	1.0	86.2
rpl32-trnL-UAG_p4	GGGATATTGGGCAGCGTTAA	GACTCGAAGTGGCAAATCCT	50.0	50.0	58.0	57.9	1056-1067 bp	1.0	86.2
rpl32-trnL-UAG_p5	GGGATATTGGGCAGCGTTAA	GCGTAAAAATAGCACTCGGC	50.0	50.0	58.0	57.9	1046 bp	0.5	59.1