Hotspot

ndhF-rpl32

SSC · 110260-110879 · 620 bp

Back to Pseudosedum

Pi 0.111

MarkerSeek score 84.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 620
variable_sites: 64
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAAGGGGAAATTGAGCCGAT	TTCGAACCGAGATGCTCAAG	50.0	50.0	57.9	58.0	2066 bp	0.5	59.5
ndhF-rpl32_p2	AACCAAAATCCCCAACACGA	TTCGAACCGAGATGCTCAAG	45.0	50.0	57.9	58.0	1892 bp	0.5	59.4
ndhF-rpl32_p3	GAAGGGGAAATTGAGCCGAT	TTAGAAGAGAATGTGCCGCC	50.0	50.0	57.9	58.0	2093 bp	0.5	59.3
ndhF-rpl32_p4	GAAGGGGAAATTGAGCCGAT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.9	58.0	1447 bp	0.5	59.3
ndhF-rpl32_p5	AACCAAAATCCCCAACACGA	TTAGAAGAGAATGTGCCGCC	45.0	50.0	57.9	58.0	1919 bp	0.5	59.2