Hotspot

psbE-petL

LSC · 63064-64099 · 1036 bp

Back to Pseudosedum

Pi 0.128

MarkerSeek score 85.80

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1036
variable_sites: 128
pi: 0.128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	CCGAAATAACCTGTTATAGTAAGCA	50.0	36.0	57.8	57.0	1089-1104 bp	1.0	50.6
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	ACCGAAATAACCTGTTATAGTAAGC	50.0	36.0	57.8	57.0	1090-1105 bp	1.0	50.6
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	CCGAAATAACCTGTTATAGTAAGCA	55.0	36.0	58.2	57.0	1084-1099 bp	1.0	50.6
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	ACCGAAATAACCTGTTATAGTAAGC	55.0	36.0	58.2	57.0	1085-1100 bp	1.0	50.6
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	CCGAAATAACCTGTTATAGTAAGCA	50.0	36.0	58.4	57.0	1082-1097 bp	1.0	50.6