Hotspot

psaA-ycf3

LSC · 41872-42532 · 661 bp

Back to Pseudosedum

Pi 0.0817

MarkerSeek score 82.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 661
variable_sites: 50
pi: 0.0817
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	722-768 bp	1.0	87.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	726-772 bp	1.0	86.9
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	721-767 bp	1.0	86.1
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TCACGAGGCGTTTCGAATAA	50.0	45.0	57.9	57.6	715-761 bp	1.0	85.7
psaA-ycf3_p5	TTGAGAAATGACCGGGTCTG	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.5	57.8	742-788 bp	1.0	84.1