Hotspot

petN-psbM

LSC · 28995-29793 · 799 bp

Back to Pseudosedum

Pi 0.101

MarkerSeek score 82.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 77
pi: 0.101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGTGTGAAAAGAGCAGGAA	CCTTCGGGTCCAAAGAAACA	50.0	50.0	58.0	58.0	2108-2156 bp	1.0	90.4
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	CCTTCGGGTCCAAAGAAACA	45.0	50.0	58.0	58.0	1546-1756 bp	1.0	89.1
petN-psbM_p3	TCTTGCTTGGGCTGCTTTAA	CATTTCACGTCAACGGGTTG	45.0	50.0	58.0	58.0	1376 bp	0.5	59.7
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TCATTTCACGTCAACGGGTT	45.0	45.0	58.0	58.0	1377 bp	0.5	59.6
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TTCATTTCACGTCAACGGGT	45.0	45.0	58.0	58.0	1378 bp	0.5	59.6