Hotspot

trnC-GCA-petN

LSC · 28065-28904 · 840 bp

Back to Pseudosedum

Pi 0.105

MarkerSeek score 76.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 840
variable_sites: 68
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	726-911 bp	1.0	90.0
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	720-905 bp	1.0	88.6
trnC-GCA-petN_p3	AGAGAGGGGGTTTTTAGGCA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.2	58.0	1121 bp	0.5	58.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTGCCCGTTCGAATTCTTT	50.0	45.0	58.1	57.8	1398 bp	0.5	57.9
trnC-GCA-petN_p5	AGTGCGAAAGGAGGCTTTTT	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	58.3	58.0	1597 bp	0.5	57.5