Hotspot

rpoB-trnC-GCA

LSC · 26870-27985 · 1116 bp

Back to Pseudosedum

Pi 0.106

MarkerSeek score 79.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 100
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTTCCCTCATTTCCATCCCG	TTCCTGCTCTTTTCACACCC	55.0	50.0	58.0	58.0	1349-1533 bp	1.0	91.0
rpoB-trnC-GCA_p2	CCAAATTAATCCCGCGGGTA	TTCCTGCTCTTTTCACACCC	50.0	50.0	58.0	58.0	1587-1771 bp	1.0	90.1
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	TTCCTGCTCTTTTCACACCC	50.0	50.0	58.0	58.0	1835-2019 bp	1.0	89.6
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	TTCCTGCTCTTTTCACACCC	50.0	50.0	58.0	58.0	2027-2211 bp	1.0	89.3
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1721-1882 bp	1.0	88.5