Hotspot

rps16-trnQ-UUG

LSC · 5883-7335 · 1453 bp

Back to Pseudosedum

Pi 0.133
MarkerSeek score 85.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1453
variable_sites
183
pi
0.133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1518-1531 bp 1.0 85.1
rps16-trnQ-UUG_p2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 1521-1534 bp 1.0 82.9
rps16-trnQ-UUG_p3 CACGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 47.6 55.0 57.5 58.1 1520-1533 bp 1.0 79.7
rps16-trnQ-UUG_p4 CACGTTGCTTTCTACCACAT GAGGTTCGAATCCTTCCGTC 45.0 55.0 56.4 58.1 1520-1533 bp 1.0 78.3
rps16-trnQ-UUG_p5 GCACGTTGCTTTCTACCAC GAGGTTCGAATCCTTCCGTC 52.6 55.0 57.3 58.1 1521-1534 bp 1.0 77.4