Hotspot

trnK-UUU-rps16

LSC · 4180-4822 · 643 bp

Back to Pseudosedum

Pi 0.107
MarkerSeek score 81.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
643
variable_sites
67
pi
0.107
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CATTTCGGATCAGTCGTGGT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1420-1444 bp 1.0 87.6
trnK-UUU-rps16_p2 CGAATCCCTTGCTTCATCCA AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1374-1398 bp 1.0 87.4
trnK-UUU-rps16_p3 ACGAATCCCTTGCTTCATCC AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1375-1399 bp 1.0 87.4
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1377-1401 bp 1.0 87.4
trnK-UUU-rps16_p5 AGCCATTCCCATTTCGGATC AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1429 bp 0.5 59.8