Hotspot

trnG-UCC

LSC · 14384-14406 · 23 bp

Back to Pseudosasa

Pi 0.0217

MarkerSeek score 53.70

Primer available yes

Label trnG-UCC

Feature Metadata

label_name: trnG-UCC
feature_type: tRNA
region: LSC
length_bp: 23
variable_sites: 1
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC_p1	AGGACGCCTTAAGTATATCA	CTCGAATGGCTACCCTTGAC	40.0	55.0	52.9	58.1	130 bp	1.0	40.6
trnG-UCC_p2	AGGACGCCTTAAGTATATCATTTCA	CTCGAATGGCTACCCTTGAC	36.0	55.0	57.9	58.1	130 bp	1.0	40.6
trnG-UCC_p3	AGGACGCCTTAAGTATATCA	CGAATGGCTACCCTTGACAA	40.0	50.0	52.9	57.9	128 bp	1.0	40.6
trnG-UCC_p4	AGGACGCCTTAAGTATATCATTTCA	CGAATGGCTACCCTTGACAA	36.0	50.0	57.9	57.9	128 bp	1.0	40.6
trnG-UCC_p5	AAGGACGCCTTAAGTATATCA	CTCGAATGGCTACCCTTGAC	38.1	55.0	53.9	58.1	131 bp	1.0	40.6