Hotspot

psbB

LSC · 71461-72987 · 1527 bp

Back to Pseudosasa

Pi 0

MarkerSeek score 38.50

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TGACACAGAATCCCCTAGAGG	AGAAAGGATGGGAGATGTTTCC	52.4	45.5	58.2	57.8	1708 bp	1.0	52.1
psbB_p2	TGACACAGAATCCCCTAGAGG	GAGTCAAAGAAAGGATGGGAGA	52.4	45.5	58.2	57.4	1715 bp	1.0	49.5
psbB_p3	TGACACAGAATCCCCTAGAGG	AGAGTCAAAGAAAGGATGGGAG	52.4	45.5	58.2	57.4	1716 bp	1.0	49.5
psbB_p4	GACACAGAATCCCCTAGAGG	AGAAAGGATGGGAGATGTTTCC	55.0	45.5	56.4	57.8	1707 bp	1.0	49.4
psbB_p5	TGACACAGAATCCCCTAGAGG	GGAGATGTTTCCCATGTCAA	52.4	45.0	58.2	55.3	1698 bp	1.0	48.6