Hotspot

ycf4-cemA

LSC · 60812-61211 · 400 bp

Back to Pseudosasa

Pi 0.00625

MarkerSeek score 59.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 400
variable_sites: 5
pi: 0.00625
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.9	57.8	504-553 bp	1.0	78.3
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.1	57.8	506-555 bp	1.0	78.0
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.8	57.8	533-582 bp	1.0	77.7
ycf4-cemA_p4	TTTCTTGCGCGTACCAATTG	AGAGAAAGAGACTCCCCAGG	45.0	55.0	57.7	57.8	509-558 bp	1.0	76.4
ycf4-cemA_p5	CCTATTTCTTGCGCGTACCA	AGAGAAAGAGACTCCCCAGG	50.0	55.0	58.4	57.8	513-562 bp	1.0	76.1