Hotspot

rbcL-psaI

LSC · 58481-59772 · 1292 bp

Back to Pseudosasa

Pi 0.00735

MarkerSeek score 51.10

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1292
variable_sites: 19
pi: 0.00735
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GCCGCAGCTTGTGAAATATG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	1382-1444 bp	1.0	82.6
rbcL-psaI_p2	GCCGCAGCTTGTGAAATATG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	1388-1450 bp	1.0	82.0
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1359-1421 bp	1.0	81.2
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1364-1426 bp	1.0	80.8
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.6	57.9	1365-1427 bp	1.0	80.6