Hotspot

trnG-UCC-trnT-GGU

LSC · 14407-16401 · 1995 bp

Back to Pseudosasa

Pi 0.0086

MarkerSeek score 42.90

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1995
variable_sites: 34
pi: 0.0086
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	TTTCCACCGAGCTGAAACAA	GCGTTCAGACCAAAAATGCA	45.0	45.0	58.0	57.9	2648-2690 bp	1.0	81.4
trnG-UCC-trnT-GGU_p2	CATCGTTAGCTTGGAAGGCT	GCGTTCAGACCAAAAATGCA	50.0	45.0	58.0	57.9	2929-2971 bp	1.0	81.3
trnG-UCC-trnT-GGU_p3	TTTCCACCGAGCTGAAACAA	TGCTTCGTTTAGCGTTCAGA	45.0	45.0	58.0	57.9	2659-2701 bp	1.0	81.3
trnG-UCC-trnT-GGU_p4	CATCGTTAGCTTGGAAGGCT	TGCTTCGTTTAGCGTTCAGA	50.0	45.0	58.0	57.9	2940-2982 bp	1.0	81.2
trnG-UCC-trnT-GGU_p5	CGTTAGCTTGGAAGGCTAGG	GCGTTCAGACCAAAAATGCA	55.0	45.0	58.1	57.9	2926-2968 bp	1.0	81.1