Hotspot

ycf3

LSC · 41828-43818 · 1991 bp

Back to Pseudoroegneria

Pi 0.000402
MarkerSeek score 58.70
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1991
variable_sites
2
pi
0.000402
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 ATCAACAACCAAACCACCCA AGCATATGGATTTAGAGCATATGGA 45.0 36.0 57.8 57.8 2151-2157 bp 1.0 40.5
ycf3_p2 TCAACAACCAAACCACCCAT AGCATATGGATTTAGAGCATATGGA 45.0 36.0 57.8 57.8 2150-2156 bp 1.0 40.5
ycf3_p3 ATCAACAACCAAACCACCCA GCATATGGATTTAGAGCATATGGA 45.0 37.5 57.8 56.5 2150-2156 bp 1.0 40.5
ycf3_p4 TCAACAACCAAACCACCCAT GCATATGGATTTAGAGCATATGGA 45.0 37.5 57.8 56.5 2149-2155 bp 1.0 40.5
ycf3_p5 TCAACAACCAAACCACCCAT AGCATATGGATTTAGAGCATATGG 45.0 37.5 57.8 56.5 2150-2156 bp 1.0 40.5