Hotspot

rpoC2

LSC · 24861-29294 · 4434 bp

Back to Pseudoroegneria

Pi 0.00108

MarkerSeek score 54.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4434
variable_sites: 12
pi: 0.00108
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AAGACGAATACAGGACCCGA	TGGGCTCCCATATTCCTCTT	50.0	50.0	58.2	57.8	119-194 bp	1.0	77.9
rpoC2_p2	AAGACGAATACAGGACCCGA	CTGGGCTCCCATATTCCTCT	50.0	55.0	58.2	58.3	120-195 bp	1.0	77.6
rpoC2_p3	GAAGACGAATACAGGACCCG	TGGGCTCCCATATTCCTCTT	55.0	50.0	57.8	57.8	120-195 bp	1.0	77.6
rpoC2_p4	AAGACGAATACAGGACCCGA	CTCTGGGCTCCCATATTCCT	50.0	55.0	58.2	58.3	122-197 bp	1.0	77.5
rpoC2_p5	AAGACGAATACAGGACCCGA	TCTGGGCTCCCATATTCCTC	50.0	55.0	58.2	58.3	121-196 bp	1.0	77.5