Hotspot

ccsA

SSC · 105588-106556 · 969 bp

Back to Pseudoroegneria

Pi 0.00124

MarkerSeek score 33.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 3
pi: 0.00124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	TCTGGACCACGAGAACTCTT	50.0	50.0	58.0	58.0	1374 bp	1.0	80.4
ccsA_p2	CTCAAGCATCTCGGTTCGAA	TTCTGGACCACGAGAACTCT	50.0	50.0	58.0	58.0	1375 bp	1.0	80.4
ccsA_p3	CTCAAGCATCTCGGTTCGAA	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	1378 bp	1.0	80.3
ccsA_p4	CTCAAGCATCTCGGTTCGAA	TGAGTGGTTTTGTCGCAGAA	50.0	45.0	58.0	58.0	1571 bp	1.0	80.1
ccsA_p5	CTCAAGCATCTCGGTTCGAA	GCCTTACCAGGAATGAGTGG	50.0	55.0	58.0	58.0	1584 bp	1.0	80.0