Hotspot

atpF

LSC · 32617-33990 · 1374 bp

Back to Pseudoroegneria

Pi 0.00117
MarkerSeek score 59.90
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1374
variable_sites
4
pi
0.00117
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 AAAAAGGGCGAGCGAAGTAA TTCGGAAGCAAGGGGAAAAA 45.0 45.0 58.1 57.9 1512-1516 bp 1.0 78.9
atpF_p2 AAAAAGGGCGAGCGAAGTAA TTTCGGAAGCAAGGGGAAAA 45.0 45.0 58.1 57.9 1513-1517 bp 1.0 78.9
atpF_p3 AAAAAGGGCGAGCGAAGTAA TTTTCGGAAGCAAGGGGAAA 45.0 45.0 58.1 57.9 1514-1518 bp 1.0 78.9
atpF_p4 AAAAAGGGCGAGCGAAGTAA TTTTTCGGAAGCAAGGGGAA 45.0 45.0 58.1 57.9 1515-1519 bp 1.0 78.9
atpF_p5 AAAAAGGGCGAGCGAAGTAA TCGAAGGGTTGCCATTAGTG 45.0 50.0 58.1 57.9 1547-1551 bp 1.0 78.6