Hotspot

psaC-ndhE

SSC · 108590-109065 · 476 bp

Back to Pseudoroegneria

Pi 0.00546

MarkerSeek score 52.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 476
variable_sites: 6
pi: 0.00546
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTTGGACAAGCTCGTACACA	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	632-633 bp	1.0	77.4
psaC-ndhE_p2	TGTTGGACAAGCTCGTACAC	AGCCGCTATTGGACTATCCA	50.0	50.0	58.1	58.3	633-634 bp	1.0	77.4
psaC-ndhE_p3	CTGTTGGACAAGCTCGTACA	AGCCGCTATTGGACTATCCA	50.0	50.0	57.9	58.3	634-635 bp	1.0	77.3
psaC-ndhE_p4	ACATCTGTTGGACAAGCTCG	AGCCGCTATTGGACTATCCA	50.0	50.0	58.2	58.3	638-639 bp	1.0	76.9
psaC-ndhE_p5	CATCTGTTGGACAAGCTCGT	AGCCGCTATTGGACTATCCA	50.0	50.0	58.2	58.3	637-638 bp	1.0	76.9