Hotspot

rpl32-trnL-UAG

SSC · 104737-105436 · 700 bp

Back to Pseudoroegneria

Pi 0.00343

MarkerSeek score 49.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 700
variable_sites: 6
pi: 0.00343
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	839-841 bp	1.0	80.2
rpl32-trnL-UAG_p2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	842-844 bp	1.0	79.7
rpl32-trnL-UAG_p3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	811-813 bp	1.0	79.5
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	810-812 bp	1.0	79.5
rpl32-trnL-UAG_p5	TTCCAGCGGTAATGAGCATC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	812-814 bp	1.0	79.2