Hotspot

rps8-rpl14

LSC · 77047-77190 · 144 bp

Back to Pseudoroegneria

Pi 0.00979

MarkerSeek score 62.20

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 144
variable_sites: 3
pi: 0.00979
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTGTCCTTGCC	CGATTGCCGAGGAATTGAGA	50.0	50.0	57.9	58.1	231-235 bp	1.0	81.0
rps8-rpl14_p2	GCAATAGTGTCCTTGCCCAT	CGATTGCCGAGGAATTGAGA	50.0	50.0	58.2	58.1	228-232 bp	1.0	80.1
rps8-rpl14_p3	TCAGCAATAGTGTCCTTGCC	GGCAATCCAAAAGGAACTCG	50.0	50.0	57.9	57.4	262-266 bp	1.0	76.1
rps8-rpl14_p4	GCAATAGTGTCCTTGCCCAT	GGCAATCCAAAAGGAACTCG	50.0	50.0	58.2	57.4	259-263 bp	1.0	75.2
rps8-rpl14_p5	TCAGCAATAGTGTCCTTGCC	AGGAACTCGAGTTTTTGGCG	50.0	50.0	57.9	58.8	251-255 bp	1.0	75.1