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psbE-petL

LSC · 62430-63475 · 1046 bp

Back to Pseudoroegneria

Pi 0.00268

MarkerSeek score 55.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 7
pi: 0.00268
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	AGGCGTATCTTGTTCAAGCC	45.0	50.0	58.0	58.3	1250-1396 bp	1.0	78.8
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGGCGTATCTTGTTCAAGCC	50.0	50.0	57.9	58.3	1239-1385 bp	1.0	78.5
psbE-petL_p3	TGACAAATAACCAACCCGCA	AAGCAGCCAGTAGAAAACCG	45.0	50.0	58.0	58.5	1204-1350 bp	1.0	77.1
psbE-petL_p4	TGACAAATAACCAACCCGCA	GCAGCCAGTAGAAAACCGAA	45.0	50.0	58.0	58.5	1202-1348 bp	1.0	77.1
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGGCGTATCTTGTTCAAGCC	50.0	50.0	58.2	58.3	1241-1387 bp	1.0	77.0