Hotspot

ndhC-trnV-UAC

LSC · 49757-50661 · 905 bp

Back to Pseudoroegneria

Pi 0.00177
MarkerSeek score 55.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
905
variable_sites
4
pi
0.00177
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCTGAAATCGAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.1 57.9 1015 bp 1.0 79.1
ndhC-trnV-UAC_p2 GCCCTGAAATCGAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.1 58.3 1016 bp 1.0 77.3
ndhC-trnV-UAC_p3 CTCTGGTCCTTCACTAACCG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.3 57.9 1044 bp 1.0 74.4
ndhC-trnV-UAC_p4 TCTGGTCCTTCACTAACCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.7 57.9 1043 bp 1.0 73.8
ndhC-trnV-UAC_p5 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 1034 bp 1.0 73.5