Hotspot

trnS-GGA-rps4

LSC · 44521-44826 · 306 bp

Back to Pseudoroegneria

Pi 0.00392

MarkerSeek score 47.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 306
variable_sites: 3
pi: 0.00392
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	ATGTAGGAGAGATGGCCGAG	ATAGGAAGTGGGTCGGTCTC	55.0	55.0	58.4	58.2	466 bp	1.0	75.8
trnS-GGA-rps4_p2	ATGTAGGAGAGATGGCCGAG	GATAGGAAGTGGGTCGGTCT	55.0	55.0	58.4	58.2	467 bp	1.0	75.8
trnS-GGA-rps4_p3	ATGTAGGAGAGATGGCCGAG	AGATAGGAAGTGGGTCGGTC	55.0	55.0	58.4	58.2	468 bp	1.0	75.7
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	GAAGTGGGTCGGTCTCAAAA	55.0	50.0	58.4	57.8	462 bp	1.0	75.5
trnS-GGA-rps4_p5	TTCGAATCCCTCTCTTTCCG	ATAGGAAGTGGGTCGGTCTC	50.0	55.0	57.0	58.2	394 bp	1.0	71.1