Hotspot

psaA-ycf3

LSC · 41124-41827 · 704 bp

Back to Pseudoroegneria

Pi 0

MarkerSeek score 53.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.1	58.0	837-865 bp	1.0	83.2
psaA-ycf3_p2	GTCTGGCCCATTCCTCAAAA	GCGCTTACTCCGGGAAATTA	50.0	50.0	58.1	58.1	833-861 bp	1.0	82.9
psaA-ycf3_p3	CTGGCCCATTCCTCAAAAGA	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	835-863 bp	1.0	82.1
psaA-ycf3_p4	TCTGGCCCATTCCTCAAAAG	TATAGCGCTTACTCCGGGAA	50.0	50.0	57.8	58.0	836-864 bp	1.0	82.0
psaA-ycf3_p5	CTGGCCCATTCCTCAAAAGA	GCGCTTACTCCGGGAAATTA	50.0	50.0	57.8	58.1	831-859 bp	1.0	81.7