Hotspot

trnfM-CAU-trnT-GGU

LSC · 12697-14737 · 2041 bp

Back to Pseudoroegneria

Pi 0.00196
MarkerSeek score 50.90
Primer available yes
Label trnfM-CAU-trnT-GGU

Feature Metadata

label_name
trnfM-CAU-trnT-GGU
feature_type
igs
region
LSC
length_bp
2041
variable_sites
9
pi
0.00196
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnfM-CAU-trnT-GGU_p1 CCTCAAGGTTATGAGCCTCG TGACTTACGTCTTAGCACGAG 55.0 47.6 57.8 57.5 2120-2125 bp 1.0 66.9
trnfM-CAU-trnT-GGU_p2 CCTCGTGAGCTACCAAACTG TGACTTACGTCTTAGCACGAG 55.0 47.6 58.3 57.5 2105-2110 bp 1.0 66.5
trnfM-CAU-trnT-GGU_p3 CCTCAAGGTTATGAGCCTCG TGACTTACGTCTTAGCACGA 55.0 45.0 57.8 56.4 2120-2125 bp 1.0 65.5
trnfM-CAU-trnT-GGU_p4 CCTCGTGAGCTACCAAACTG TGACTTACGTCTTAGCACGA 55.0 45.0 58.3 56.4 2105-2110 bp 1.0 65.1
trnfM-CAU-trnT-GGU_p5 CCTCAAGGTTATGAGCCTCG GACTTACGTCTTAGCACGAGA 55.0 47.6 57.8 57.3 2119-2124 bp 1.0 64.8