Hotspot

psbK-psbI

LSC · 6929-7333 · 405 bp

Back to Pseudoroegneria

Pi 0.0079

MarkerSeek score 56.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 405
variable_sites: 8
pi: 0.0079
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCGCGTCCAG	50.0	50.0	57.9	57.9	539-549 bp	1.0	81.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCCGTCCTGGGTCATTAGA	50.0	50.0	57.9	57.8	517-527 bp	1.0	80.4
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	536-546 bp	1.0	79.6
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	537-547 bp	1.0	79.6
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCGCGTCCAG	45.0	50.0	58.3	57.9	538-548 bp	1.0	79.6