Hotspot

tufA

SSC · 139032-140288 · 1257 bp

Back to Pseudopediastrum

Pi 0.472

MarkerSeek score 82.90

Primer available yes

Label tufA

Feature Metadata

label_name: tufA
feature_type: gene
region: SSC
length_bp: 1257
variable_sites: 548
pi: 0.472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tufA_p1	CAAAGCGAAGAAGAAGCAGC	CTTCCTGTCTTCGCTTCCTC	50.0	55.0	58.0	58.0	2013 bp	0.5	59.7
tufA_p2	GAAGAAGCAGCAAAAGAGCG	CTTCCTGTCTTCGCTTCCTC	50.0	55.0	58.0	58.0	2004 bp	0.5	59.7
tufA_p3	GCAAAGCGAAGAAGAAGCAG	CTTCCTGTCTTCGCTTCCTC	50.0	55.0	58.0	58.0	2014 bp	0.5	59.7
tufA_p4	CAAAGCGAAGAAGAAGCAGC	AGGAGGCAAAGCGAAAAAGA	50.0	45.0	58.0	58.0	2451 bp	0.5	59.5
tufA_p5	GAAGAAGCAGCAAAAGAGCG	AGGAGGCAAAGCGAAAAAGA	50.0	45.0	58.0	58.0	2442 bp	0.5	59.5