Hotspot

rps18

SSC · 150451-150876 · 426 bp

Back to Pseudopediastrum

Pi 0.507

MarkerSeek score 78.50

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: SSC
length_bp: 426
variable_sites: 194
pi: 0.507
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	TGGGCCATCTACAATTGACG	CGAGGAGGAAGATGCATAGC	50.0	55.0	58.0	57.9	1378 bp	0.5	59.1
rps18_p2	TGGGCCATCTACAATTGACG	GCGAGGAGGAAGATGCATAG	50.0	55.0	58.0	57.9	1379 bp	0.5	59.1
rps18_p3	TGGGCCATCTACAATTGACG	CAAAGCGAAGAGGAAGCTCT	50.0	50.0	58.0	57.9	1415 bp	0.5	59.0
rps18_p4	TGGGCCATCTACAATTGACG	AACTTGTTAACTCGCGCTCT	50.0	45.0	58.0	57.8	1438 bp	0.5	58.5
rps18_p5	TGGGCCATCTACAATTGACG	ACTTGTTAACTCGCGCTCTT	50.0	45.0	58.0	57.8	1437 bp	0.5	58.5