Hotspot

clpP

SSC · 152053-153675 · 1623 bp

Back to Pseudopediastrum

Pi 0.458

MarkerSeek score 82.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: SSC
length_bp: 1623
variable_sites: 668
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGCACTCTCCTTCAGTCTGA	GTGGTGAAGGAGGAAAAGCA	50.0	50.0	58.0	58.0	2059 bp	0.5	59.8
clpP_p2	TGCACTCTCCTTCAGTCTGA	TTCTTCGTGTACAGAGCGTG	50.0	50.0	58.0	58.0	2076 bp	0.5	59.6
clpP_p3	TGCACTCTCCTTCAGTCTGA	TCCTTCTTCTTCGCTGCTTC	50.0	50.0	58.0	57.9	2575 bp	0.5	59.3
clpP_p4	TGCACTCTCCTTCAGTCTGA	CCTTCTTCTTCGCTGCTTCT	50.0	50.0	58.0	57.9	2574 bp	0.5	59.3
clpP_p5	TGCACTCTCCTTCAGTCTGA	CTTCCTTCTTCTTCGCTGCT	50.0	50.0	58.0	57.9	2577 bp	0.5	59.3