Hotspot

atpB

SSC · 136855-138300 · 1446 bp

Back to Pseudopediastrum

Pi 0.486

MarkerSeek score 80.00

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: SSC
length_bp: 1446
variable_sites: 576
pi: 0.486
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	ATAGGGTTTTTGGTGCGTGT	CGCTCTTTTGCTGCTTCTTC	45.0	50.0	58.0	58.0	1991 bp	0.5	59.6
atpB_p2	ATAGGGTTTTTGGTGCGTGT	CTGCTTCTTCTTCGCTTTGC	45.0	50.0	58.0	58.0	1981 bp	0.5	59.6
atpB_p3	ATAGGGTTTTTGGTGCGTGT	GCTGCTTCTTCTTCGCTTTG	45.0	50.0	58.0	58.0	1982 bp	0.5	59.6
atpB_p4	TGGTGCGTGTTAGCTTCTTT	CTGCTTCTTCTTCGCTTTGC	45.0	50.0	58.0	58.0	1971 bp	0.5	59.4
atpB_p5	TGGTGCGTGTTAGCTTCTTT	GCTGCTTCTTCTTCGCTTTG	45.0	50.0	58.0	58.0	1972 bp	0.5	59.4