Hotspot

trnS(gcu)-DR615_pgr003

IRa · 190330-191045 · 716 bp

Back to Pseudopediastrum

Pi 0.355

MarkerSeek score 86.50

Primer available yes

Label trnS(gcu)-DR615_pgr003

Feature Metadata

label_name: trnS(gcu)-DR615_pgr003
feature_type: igs
region: IRa
length_bp: 716
variable_sites: 207
pi: 0.355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 86.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS(gcu)-DR615_pgr003_p1	CGGATTAGCAATCAGTCGCT	GTTGGAAGTCTGGCAGGAAA	50.0	50.0	58.1	58.0	799-971 bp	1.0	90.7
trnS(gcu)-DR615_pgr003_p2	CGGATTAGCAATCAGTCGCT	TGGAAGTCTGGCAGGAAAAC	50.0	50.0	58.1	58.0	797-969 bp	1.0	90.7
trnS(gcu)-DR615_pgr003_p3	CGGATTAGCAATCAGTCGCT	ACTTGTTGGAAGTCTGGCAG	50.0	50.0	58.1	58.0	803-975 bp	1.0	90.6
trnS(gcu)-DR615_pgr003_p4	CGGATTAGCAATCAGTCGCT	GACTTGTTGGAAGTCTGGCA	50.0	50.0	58.1	58.0	804-976 bp	1.0	90.6
trnS(gcu)-DR615_pgr003_p5	CGGATTAGCAATCAGTCGCT	AGGAAAACTCAACTAGCGCC	50.0	50.0	58.1	58.2	785-957 bp	1.0	89.3