Hotspot

rpl5-rpl14

SSC · 178765-179681 · 917 bp

Back to Pseudopediastrum

Pi 0.375
MarkerSeek score 85.10
Primer available yes
Label rpl5-rpl14

Feature Metadata

label_name
rpl5-rpl14
feature_type
igs
region
SSC
length_bp
917
variable_sites
316
pi
0.375
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl5-rpl14_p1 TTGTTTTAAACGCTGAGTCA TGTTTCTTTAGCACCAGAAGT 35.0 38.1 53.7 55.4 966-1149 bp 1.0 52.0
rpl5-rpl14_p2 TTGTTTTAAACGCTGAGTCA TTGTTTCTTTAGCACCAGAAGT 35.0 36.4 53.7 56.1 967-1150 bp 1.0 52.0
rpl5-rpl14_p3 TGTTTTAAACGCTGAGTCAT TGTTTCTTTAGCACCAGAAGT 35.0 38.1 53.5 55.4 965-1148 bp 1.0 52.0
rpl5-rpl14_p4 ATTGTTTTAAACGCTGAGTCA TGTTTCTTTAGCACCAGAAGT 33.3 38.1 54.3 55.4 967-1150 bp 1.0 52.0
rpl5-rpl14_p5 TTGTTTTAAACGCTGAGTCAT TGTTTCTTTAGCACCAGAAGT 33.3 38.1 54.3 55.4 966-1149 bp 1.0 52.0