Hotspot

psaA-rps8

SSC · 176441-176999 · 559 bp

Back to Pseudopediastrum

Pi 0.108

MarkerSeek score 83.90

Primer available yes

Label psaA-rps8

Feature Metadata

label_name: psaA-rps8
feature_type: igs
region: SSC
length_bp: 559
variable_sites: 58
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-rps8_p1	TTTTAGCTTCGCGTTCTGGT	TGGGGGTGAAGTTTTATGTTCA	45.0	40.9	58.1	57.8	624-1060 bp	1.0	69.0
psaA-rps8_p2	CACACGCCCATTTTTCGAAA	TGGGGGTGAAGTTTTATGTTCA	45.0	40.9	57.9	57.8	684-1120 bp	1.0	68.5
psaA-rps8_p3	ACACGCCCATTTTTCGAAAC	TGGGGGTGAAGTTTTATGTTCA	45.0	40.9	57.9	57.8	683-1119 bp	1.0	68.5
psaA-rps8_p4	CACGCCCATTTTTCGAAACT	TGGGGGTGAAGTTTTATGTTCA	45.0	40.9	57.6	57.8	682-1118 bp	1.0	66.2
psaA-rps8_p5	TCACACGCCCATTTTTCGAA	TGGGGGTGAAGTTTTATGTTCA	45.0	40.9	58.7	57.8	685-1121 bp	1.0	64.0