Hotspot

psbD-psaA

LSC · 70346-71442 · 1097 bp

Back to Pseudopediastrum

Pi 0.225

MarkerSeek score 83.20

Primer available yes

Label psbD-psaA

Feature Metadata

label_name: psbD-psaA
feature_type: igs
region: LSC
length_bp: 1097
variable_sites: 202
pi: 0.225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-psaA_p1	CAAAACGGTCTTGACGAAGC	TGGTCATTCTTCTTAGCACGT	50.0	42.9	58.0	57.3	1209-1221 bp	1.0	78.4
psbD-psaA_p2	GAAGCCAGTCATCTGCATCA	TGGTCATTCTTCTTAGCACGT	50.0	42.9	58.0	57.3	1194-1206 bp	1.0	78.3
psbD-psaA_p3	GCCAGTCATCTGCATCATCA	TGGTCATTCTTCTTAGCACGT	50.0	42.9	58.0	57.3	1191-1203 bp	1.0	78.3
psbD-psaA_p4	TTGACGAAGCCAGTCATCTG	TGGTCATTCTTCTTAGCACGT	50.0	42.9	57.9	57.3	1199-1211 bp	1.0	77.9
psbD-psaA_p5	GTCTTGACGAAGCCAGTCAT	TGGTCATTCTTCTTAGCACGT	50.0	42.9	57.9	57.3	1202-1214 bp	1.0	77.9