Hotspot

psbF-psbL

LSC · 58139-58482 · 344 bp

Back to Pseudopediastrum

Pi 0.0901
MarkerSeek score 83.20
Primer available yes
Label psbF-psbL

Feature Metadata

label_name
psbF-psbL
feature_type
igs
region
LSC
length_bp
344
variable_sites
31
pi
0.0901
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbL_p1 CATGCATTAGCTGTTCCAACA ATTGGGATTAGGTCTAGCCA 42.9 45.0 57.1 55.3 434-464 bp 1.0 48.8
psbF-psbL_p2 CATGCATTAGCTGTTCCAACA TTGGGATTAGGTCTAGCCAT 42.9 45.0 57.1 55.3 433-463 bp 1.0 48.8
psbF-psbL_p3 TCATGCATTAGCTGTTCCAAC ATTGGGATTAGGTCTAGCCA 42.9 45.0 56.9 55.3 435-465 bp 1.0 46.6
psbF-psbL_p4 TCATGCATTAGCTGTTCCAAC TTGGGATTAGGTCTAGCCAT 42.9 45.0 56.9 55.3 434-464 bp 1.0 46.6
psbF-psbL_p5 CATGCATTAGCTGTTCCAACA CCCCAATAAAGACTAGTACGGT 42.9 45.5 57.1 57.0 475-505 bp 1.0 46.1