Hotspot

rpoBa-psbF

LSC · 55893-58003 · 2111 bp

Back to Pseudopediastrum

Pi 0.134
MarkerSeek score 85.70
Primer available yes
Label rpoBa-psbF

Feature Metadata

label_name
rpoBa-psbF
feature_type
igs
region
LSC
length_bp
2111
variable_sites
201
pi
0.134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.71
markerseek_score
85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoBa-psbF_p1 TGCATGTCCACAAAATCTGG TGTTGGAACAGCTAATGCATG 45.0 42.9 56.6 57.1 2110-2248 bp 1.0 65.3
rpoBa-psbF_p2 GCATGTCCACAAAATCTGGT TGTTGGAACAGCTAATGCATG 45.0 42.9 56.6 57.1 2109-2247 bp 1.0 65.3
rpoBa-psbF_p3 TGCATGTCCACAAAATCTGGT TGTTGGAACAGCTAATGCATG 42.9 42.9 58.4 57.1 2110-2248 bp 1.0 65.3
rpoBa-psbF_p4 TTGCATGTCCACAAAATCTGG TGTTGGAACAGCTAATGCATG 42.9 42.9 57.3 57.1 2111-2249 bp 1.0 63.2
rpoBa-psbF_p5 TGCATGTCCACAAAATCTGG GTTGGAACAGCTAATGCATGA 45.0 42.9 56.6 56.9 2109-2247 bp 1.0 63.1