Hotspot

rpl32-ndhF

SSC · 125031-126131 · 1101 bp

Back to Pseudognaphalium

Pi 0.0137

MarkerSeek score 68.10

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1101
variable_sites: 28
pi: 0.0137
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1252-1296 bp	1.0	84.4
rpl32-ndhF_p2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1241-1285 bp	1.0	84.4
rpl32-ndhF_p3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1250-1294 bp	1.0	84.3
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	45.0	45.0	57.9	58.0	1251-1295 bp	1.0	84.3
rpl32-ndhF_p5	AACGCTGCCCAATATCCTTT	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1239-1283 bp	1.0	84.3